Advances in Lensless Digital Holographic Microscopy for Biomedical Applications

Optical microscopy, fundamental in biomedical research, faces limitations such as phototoxicity and alteration of physiological processes due to fluorescence labeling. Quantitative Phase Microscopy (QPM) has emerged as a high-contrast, label-free imaging technique, yet its benchtop variants are often bulky and expensive. Lensless holographic microscopy (LHM) offers a cost-effective and simple alternative, capturing large volumes without the depth of focus and field of view limitations inherent in traditional microscopy. In this talk, I will present new experimental and computational strategies for enhancing signal-to-noise ratio in LHM, enabling high-throughput, marker-free analysis of dynamic biological processes in challenging conditions, with applications including brain tissue imaging and live cell migration studies.